How it works
Where to file your trade vs. where the trade licensing office sits
A trade licensing office does not sit in every municipality — there are 227. But you can file your registration almost anywhere. Here is the difference.
Where the office actually sits (227 places)
The trade-licensing agenda is handled only by municipal trade licensing offices — departments of the municipalities with extended powers (ORP). There are 205 of them plus 22 in Prague, 227 in total. Above them sit 14 regional offices as the appellate body. Ordinary municipal offices and authorised municipal offices have no trade licensing office.
Where you can file (almost anywhere)
You need not take your registration to "your" office. Any of the 227 municipal trade licensing offices will accept it (they act as a central registration point). In addition, any Czech POINT contact point (~7,000 places — municipal offices, post offices, notaries) will take the paperwork and forward it to the trade licensing office under Section 72 of the Trade Licensing Act. Czech POINT itself does not decide on the trade, though.
Mind the names
A "municipal office" (the town hall in every municipality) is not the same as a "municipal trade licensing office" (the ORP level, 227 places). Two words apart, but a whole tier of authority apart.